ELISA/assay

Human protein disulfide-isomerase A3, PDIA3 ELISA Kit

MBS700585

48-Strip-Wells

565 USD

ELISA Kit

Human protein disulfide-isomerase A3, PDIA3 ELISA Kit

protein disulfide isomerase family A, member 3

Human protein disulfide-isomerase A3; PDIA3 ELISA Kit; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; 58 kDa microsomal protein; endoplasmic reticulum P58; glucose regulated protein; 58kDa; phospholipase C-alpha; protein disulfide isomerase; protein disulfide isomerase family A; member 3

protein disulfide-isomerase A3; Protein disulfide-isomerase A3; protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n; protein disulfide isomerase family A, member 3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60

PDIA3

PDIA3; PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n; ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60

PDIA3_HUMAN

Human

505

This assay has high sensitivity and excellent specificity for detection of human PDIA3. No significant cross-reactivity or interference between human PDIA3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml -80 ng/ml. Sensitivity: The minimum detectable dose of human PDIA3 is typically less than 0.31 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDIA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDIA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

21361657

NP_005304.3

NM_005313.4

P30101

56,782 Da

Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Asparagine N-linked Glycosylation Pathway (161013); Calnexin/calreticulin Cycle Pathway (187218); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); ER-Phagosome Pathway (477124); Immune System Pathway (106386)

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway. Protein type: EC 5.3.4.1; Endoplasmic reticulum; Isomerase; Protease; Apoptosis; Chaperone; Phospholipase. Chromosomal Location of Human Ortholog: 15q15. Cellular Component: focal adhesion; cell surface; smooth endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus. Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity. Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150