ELISA/assay

Rat heat shock protein 60, hSP-60 ELISA Kit

MBS700577

96 Strip Wells

915 USD

ELISA Kit

Rat heat shock protein 60, hSP-60 ELISA Kit

heat shock 60kDa protein 1 (chaperonin)

Rat heat shock protein 60; hSP-60 ELISA Kit; CPN60; GROEL; HLD4; HSP60; HSP65; HuCHA60; SPG13; P60 lymphocyte protein; chaperonin; heat shock protein 65; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1; heat shock 60kDa protein 1 (chaperonin)

60 kDa heat shock protein, mitochondrial; 60 kDa heat shock protein, mitochondrial; 60 kDa heat shock protein, mitochondrial; CPN60; HSP-60; HSP-65; chaperonin 60; 60 kDa chaperonin; heat shock 60kDa protein 1; heat shock protein 60 (liver); mitochondrial matrix protein P1; heat shock 60kD protein 1 (chaperonin); heat shock protein 1 (chaperonin); 60 kDa chaperonin; Chaperonin 60; CPN60; HSP-65; Heat shock protein 60; HSP-60; Hsp60; Mitochondrial matrix protein P1

HSPD1

Hspd1; Hspd1; Hsp60; Hspd1-30p; Hsp60; CPN60; HSP-60; Hsp60

CH60_RAT

Rat

573

This assay has high sensitivity and excellent specificity for detection of Rat HSPD1. No significant cross-reactivity or interference between Rat HSPD1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-60. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSP-60 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HSP-60, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HSP-60 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

206597443

NP_071565.2

NM_022229.2

P63039

60,955 Da

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a stress-inducible molecular chaperone which participates in protein folding [RGD, Feb 2006]

Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.UniRule annotationHAMAP-Rule:MF_00600

96 Strip Wells

915 USD