ELISA/assay

Mouse cluster Of differentiation, CD33 ELISA Kit

MBS700576

96 Strip Wells

850 USD

ELISA Kit

Mouse cluster Of differentiation, CD33 ELISA Kit

CD33 molecule

Mouse cluster Of differentiation (CD33) ELISA Kit; FLJ00391; SIGLEC-3; SIGLEC3; p67; CD33 antigen; CD33 antigen (gp67) ; CD33 molecule

myeloid cell surface antigen CD33 isoform 1; Myeloid cell surface antigen CD33; myeloid cell surface antigen CD33; sialic acid-binding Ig-like lectin 3; CD33 antigen; Sialic acid-binding Ig-like lectin 3; Siglec-3; CD_antigen: CD33

CD33

Cd33; Cd33; gp67; Siglec-3; Siglec3; Siglec-3

CD33_MOUSE

Mouse

403

This assay has high sensitivity and excellent specificity for detection of Mouse CD33. No significant cross-reactivity or interference between Mouse CD33 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD33. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CD33 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CD33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CD33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

161484603

NP_001104528.1

NM_001111058.1

Q63994

44,824 Da

Hematopoietic Cell Lineage Pathway 83275!!Hematopoietic Cell Lineage Pathway 489

Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

96 Strip Wells

850 USD