ELISA/assay

Rat Caspase 3, Casp-3 ELISA Kit

MBS700575

48-Strip-Wells

465 USD

ELISA Kit

Rat Caspase 3, Casp-3 ELISA Kit

caspase 3, apoptosis-related cysteine peptidase

Rat Caspase 3; Casp-3 ELISA Kit; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; caspase 3; caspase 3; apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; caspase 3; apoptosis-related cysteine peptidase

caspase-3; Caspase-3; caspase-3; CASP-3; apopain; cysteine protease CPP32; Apopain; Cysteine protease CPP32Cleaved into the following 2 chains:Caspase-3 subunit p17; Caspase-3 subunit p12

CASP3

CASP3; CASP3; CASP-3

CASP3_RABIT

Rat

277

This assay has high sensitivity and excellent specificity for detection of rat Casp-3. No significant cross-reactivity or interference between rat Casp-3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat Casp-3 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Casp-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Casp-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Casp-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Casp-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

126723382

NP_001075586.1

NM_001082117.1

Q8MJC3

31,653 Da

Activation Of DNA Fragmentation Factor Pathway (105683); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198806)

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage . Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595