Mouse myelin basic protein, MBP ELISA Kit | MyBioSource MBS700572 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse myelin basic protein, MBP ELISA Kit

catalog :

MBS700572

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700572

products type :

ELISA Kit

products full name :

Mouse myelin basic protein, MBP ELISA Kit

products short name :

myelin basic protein

products name syn :

Mouse myelin basic protein; MBP ELISA Kit; MGC99675; Golli-mbp; OTTHUMP00000174383; myelin basic protein

other names :

Golli-Mbp isoform 2; Myelin basic protein; Golli-Mbp; myelin basic protein; shiverer; myelin deficient; myelin A1 protein; myelin basic protein; Myelin A1 protein

products gene name :

MBP

other gene names :

Mbp; Mbp; mld; shi; Hmbpr; C76307; R75289; golli-mbp; Shi; MBP

uniprot entry name :

MBP_MOUSE

reactivity :

Mouse

sequence length :

195

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse MBP. No significant cross-reactivity or interference between mouse MBP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: The minimum detectable dose of mouse MBP is typically less than 4.69 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

69885018

ncbi acc num :

NP_001020416.1

ncbi gb acc num :

NM_001025245.1

uniprot acc num :

P04370

ncbi mol weight :

27,168 Da

ncbi pathways :

MAPK Cascade Pathway (198366); Spinal Cord Injury Pathway (739004)

ncbi summary :

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.1 PublicationManual assertion based on experiment in:Ref.17

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage