ELISA/assay

Rat fibroblast growth factor 23, FGF23 ELISA Kit

MBS700554

48-Strip-Wells

565 USD

ELISA Kit

Rat fibroblast growth factor 23, FGF23 ELISA Kit

fibroblast growth factor 23

Rat fibroblast growth factor 23 (FGF23) ELISA Kit; ADHR; HPDR2; HYPF; PHPTC; tumor-derived hypophosphatemia inducing factor; fibroblast growth factor 23

fibroblast growth factor 23; Fibroblast growth factor 23; fibroblast growth factor 23; FGF-23; fibroblast growth factor 23

FGF23

Fgf23; Fgf23; FGF-23

FGF23_RAT

Rat

251

This assay has high sensitivity and excellent specificity for detection of rat FGF23. No significant cross-reactivity or interference between rat FGF23 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml-100 pg/ml. Sensitivity: The minimum detectable dose of rat FGF23 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF23 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF23 bound in the initial step. The color development is stopped and the intensity of the color is measured.

18543369

NP_570110.1

NM_130754.1

Q8VI82

27,912 Da

Activated Point Mutants Of FGFR2 Pathway (1010497); Adaptive Immune System Pathway (1010925); Constitutive PI3K/AKT Signaling In Cancer Pathway (1010535); DAP12 Interactions Pathway (1011029); DAP12 Signaling Pathway (1011030); Disease Pathway (1010484); Downstream Signal Transduction Pathway (1010117); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1010939); Downstream Signaling Of Activated FGFR Pathway (1010048); FGFR Ligand Binding And Activation Pathway (1010034)

Function: Regulator of phosphate homeostasis Inhibits renal tubular phosphate transport by reducing SLC34A1 levels Regulator of vitamin-D metabolism Negatively regulates osteoblasts differentiation and matrix mineralization Acts directly on the parathyroid to decrease PTH secretion. Upregulates EGR1 expression in the presence of KL.2 PublicationsManual assertion based on experiment in:Ref.2

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150