Human alpha2-Antiplasmin, alpha2-AP ELISA Kit | MyBioSource MBS700548 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human alpha2-Antiplasmin, alpha2-AP ELISA Kit

catalog :

MBS700548

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700548

products type :

ELISA Kit

products full name :

Human alpha2-Antiplasmin, alpha2-AP ELISA Kit

products short name :

serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor) , member 2

products name syn :

Human alpha2-Antiplasmin; alpha2-AP ELISA Kit; A2AP; AAP; ALPHA-2-PI; API; PLI; OTTHUMP00000115656; alpha-2-antiplasmin; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived; serpin peptidase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived factor); member 2

other names :

alpha-2-antiplasmin isoform a; Alpha-2-antiplasmin; alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2

products gene name :

SERPINF2

other gene names :

SERPINF2; SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI; AAP; PLI; Alpha-2-AP; Alpha-2-PI

uniprot entry name :

A2AP_HUMAN

reactivity :

Human

sequence length :

491

specificity :

This assay has high sensitivity and excellent specificity for detection of human alpha2 -AP. No significant cross-reactivity or interference between human alpha2 -AP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human alpha2 -AP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alpha2-AP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alpha2-AP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alpha2-AP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alpha2-AP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

115583663

ncbi acc num :

NP_000925.2

ncbi gb acc num :

NM_000934.3

uniprot acc num :

P08697

ncbi mol weight :

54,566 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

uniprot summary :

Function: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.1 PublicationManual assertion based on experiment in:Ref.15

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage