catalog number :
MBS700543
products type :
ELISA Kit
products full name :
Human Tartrate Resistant Acid Phosphatase, TRAP ELISA Kit
products short name :
Tartrate Resistant Acid Phosphatase (TRAP)
products name syn :
Human Tartrate Resistant Acid Phosphatase (TRAP) ELISA Kit; Tartrate Resistant Acid Phosphatase (TRAP)
other names :
traP; Protein TraP
products gene name :
TRAP
other gene names :
traP; ECOK12F079
uniprot entry name :
TRAP_ECOLI
specificity :
This assay has high sensitivity and excellent specificity for detection of human TRAP. No significant cross-reactivity or interference between human TRAP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.47 ng/ml -30 ng/ml. Sensitivity: 0.11 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
BAA97949.1
ncbi mol weight :
21,961 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells