ELISA/assay

Pig intestinal fatty acid binding protein, iFABP ELISA Kit

MBS700522

96 Strip Wells

895 USD

ELISA Kit

Pig intestinal fatty acid binding protein, iFABP ELISA Kit

fatty acid binding protein 2, intestinal

Pig intestinal fatty acid binding protein; iFABP ELISA Kit; FABPI; I-FABP; MGC133132; intestinal fatty acid binding protein 2; fatty acid binding protein 2; intestinal

fatty acid-binding protein, intestinal; Fatty acid-binding protein, intestinal; fatty acid-binding protein, intestinal; fatty acid-binding protein 2; intestinal fatty acid binding protein; intestinal-type fatty acid-binding protein; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP

FABP2

FABP2; FABP2; I-FABP; FABPI; I-FABP

FABPI_PIG

Pig

132

This assay has high sensitivity and excellent specificity for detection of Pig FABP2. No significant cross-reactivity or interference between Pig FABP2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 78 pg/ml-5000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 19.5 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for iFABP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any iFABP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for iFABP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of iFABP bound in the initial step. The color development is stopped and the intensity of the color is measured.

72535172

NP_001026950.1

NM_001031780.1

Q45KW7

15,217 Da

Fat Digestion And Absorption Pathway 194378!!Fat Digestion And Absorption Pathway 194324!!PPAR Signaling Pathway 84457!!PPAR Signaling Pathway 450

Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor

96 Strip Wells

895 USD