ELISA/assay

Human cardiac troponin T, cTn-T ELISA Kit

MBS700509

48-Strip-Wells

565 USD

ELISA Kit

Human cardiac troponin T, cTn-T ELISA Kit

troponin T type 1 (skeletal, slow)

Human cardiac troponin T (cTn-T) ELISA Kit; ANM; FLJ98147; MGC104241; STNT; TNT; TNTS; slow skeletal muscle troponin T; troponin T1; skeletal; slow; troponin-T1; skeletal; slow; troponin T type 1 (skeletal; slow)

troponin T, slow skeletal muscle isoform b; Troponin T, slow skeletal muscle; troponin T, slow skeletal muscle; troponin-T1, skeletal, slow; slow skeletal muscle troponin T; troponin T type 1 (skeletal, slow); Slow skeletal muscle troponin T; sTnT

TNNT1

TNNT1; TNNT1; ANM; TNT; NEM5; STNT; TNTS; TNT; TnTs; sTnT

TNNT1_HUMAN

Human

262

This assay has high sensitivity and excellent specificity for detection of Human TNNT1. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cTn-T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTn-T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-T is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-T bound in the initial step. The color development is stopped and the intensity of the color is measured.

187173290

NP_001119604.1

NM_001126132.2

P13805

32,948 Da

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150