ELISA/assay

Human Parathyroid Hormone Related Protein, PTHrP ELISA Kit

MBS700500

96 Strip Wells

850 USD

ELISA Kit

Human Parathyroid Hormone Related Protein, PTHrP ELISA Kit

parathyroid hormone-like hormone

Human Parathyroid Hormone Related Protein; PTHrP ELISA Kit; HHM; MGC14611; PLP; PTHR; PTHRP; PTH-related protein; humoral hypercalcemia of malignancy; osteostatin; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-rel; parathyroid hormone-like hormone

parathyroid hormone-related protein isoform 2 preproprotein; Parathyroid hormone-related protein; parathyroid hormone-related protein; PTH-rP; osteostatin; PTH-related protein; parathyroid hormone-like related protein; parathyroid hormone-like hormone preproprotein; parathyroid hormone-like hormone; Parathyroid hormone-like protein; PLP

PTHLH

PTHLH; PTHLH; HHM; PLP; BDE2; PTHR; PTHRP; PTHRP; PTH-rP; PTHrP; PLP

PTHR_HUMAN

Human

175

This assay has high sensitivity and excellent specificity for detection of Human PTHLH. No significant cross-reactivity or interference between Human PTHLH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 31.25 pg/ml-2000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 7.81 pg/ml

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTHrP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTHrP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTHrP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTHrP bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506269

NP_002811.1

NM_002820.2

P12272

20,194 Da

Class B/2 (Secretin Family Receptors) Pathway 106378!!Endochondral Ossification Pathway 198812!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356!!Signaling Events Mediated By The Hedgehog Family Pathway 137950

The protein encoded by this gene is a member of the parathyroid hormone family. This hormone, via its receptor, PTHR1, regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. It is responsible for most cases of humoral hypercalcemia of malignancy, and mutations in this gene are associated with brachydactyly type E2 (BDE2). Alternatively spliced transcript variants have been found for this gene. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, downstream of the initiator AUG codon, resulting in nuclear forms of this hormone. [provided by RefSeq, Nov 2013]

Function: Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1.1 PublicationManual assertion based on experiment in:Ref.18

96 Strip Wells

850 USD