ELISA/assay

Human eosinophil Cationic protein, ECP ELISA Kit

MBS700481

48-Strip-Wells

565 USD

ELISA Kit

Human eosinophil Cationic protein, ECP ELISA Kit

eosinophil cationic protein, ECP

Human eosinophil cationic protein; ECP ELISA Kit; eosinophil cationic protein; ECP

eosinophil cationic protein; Eosinophil cationic protein; eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease; ribonuclease, RNase A family, 3; Ribonuclease 3; RNase 3

ECP

RNASE3; RNASE3; ECP; RNS3; ECP; RNS3; ECP; RNase 3

ECP_HUMAN

Human

160

This assay has high sensitivity and excellent specificity for detection of human ECP. No significant cross-reactivity or interference between human ECP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: 0.39 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ECP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ECP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECP bound in the initial step. The color development is stopped and the intensity of the color is measured.

45243507

NP_002926.2

NM_002935.2

P12724

18,385 Da

Asthma Pathway (83120); Asthma Pathway (532)

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Function: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.2 PublicationsManual assertion based on experiment in:Ref.10

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150