ELISA/assay

Human G protein-coupled bile acid receptor 1, GPBAR1 ELISA Kit

MBS700478

96 Strip Wells

850 USD

ELISA Kit

Human G protein-coupled bile acid receptor 1, GPBAR1 ELISA Kit

G protein-coupled bile acid receptor 1

Human G protein-coupled bile acid receptor 1 (GPBAR1) ELISA Kit; BG37; GPCR; GPCR19; GPR131; M-BAR; MGC40597; TGR5; G-protein coupled bile acid receptor BG37; membrane bile acid receptor; membrane-type receptor for bile acids; G protein-coupled bile acid receptor 1

G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor 1; hBG37; hGPCR19; membrane bile acid receptor; G-protein coupled receptor GPCR19; membrane-type receptor for bile acids; G-protein coupled bile acid receptor BG37; G protein-coupled bile acid receptor 1; G-protein coupled receptor GPCR19; hGPCR19; Membrane-type receptor for bile acids; M-BAR; hBG37; BG37

GPBAR1

GPBAR1; GPBAR1; BG37; TGR5; M-BAR; GPCR19; GPR131; TGR5; hGPCR19; M-BAR; BG37

GPBAR_HUMAN

Human

330

This assay has high sensitivity and excellent specificity for detection of Human GPBAR1. No significant cross-reactivity or interference between Human GPBAR1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPBAR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPBAR1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GPBAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GPBAR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

116284384

NP_001070659.1

NM_001077191.1

Q8TDU6

35,248 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Function: Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.2 PublicationsManual assertion based on experiment in:Ref.1

96 Strip Wells

850 USD