catalog number :
MBS700477
products type :
ELISA Kit
products full name :
Dog corticotropin releasing hormone, CRH ELISA Kit
products short name :
corticotropin releasing hormone
products name syn :
Dog corticotropin releasing hormone; CRH ELISA Kit; CRF; corticotropin-releasing factor; corticotropin releasing hormone
other names :
Corticoliberin; Corticoliberin; corticoliberin; CRF; corticotropin-releasing factor; corticotropin-releasing hormone; Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone
other gene names :
CRH; CRH; CRF
uniprot entry name :
CRF_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog CRH. No significant cross-reactivity or interference between Dog CRH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 7.81 pg/ml-500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 1.95 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
21,547 Da
ncbi pathways :
Alcoholism Pathway 585569!!Alcoholism Pathway 587116!!Class B/2 (Secretin Family Receptors) Pathway 1017214!!G Alpha (s) Signalling Events Pathway 1017219!!GPCR Downstream Signaling Pathway 1017218!!GPCR Ligand Binding Pathway 1017188!!Long-term Depression Pathway 84136!!Long-term Depression Pathway 497!!Signal Transduction Pathway 1017095!!Signaling By GPCR Pathway 1017187
uniprot summary :
Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.