ELISA/assay

Human beta-defensins 3 ELISA Kit

MBS700471

48-Strip-Wells

510 USD

ELISA Kit

Human beta-defensins 3 ELISA Kit

defensin, beta 103A

Human beta-defensins 3 ELISA Kit; DEFB103; DEFB3; HBD-3; HBD3; HBP-3; HBP3; beta-defensin 3; defensin; beta 3; defensin; beta 103A

beta-defensin 103; Beta-defensin 103; beta-defensin 103; beta-defensin 3; defensin, beta 3; defensin, beta 103; defensin-like protein; defensin, beta 103A; Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein

DEFB103A

DEFB103A; DEFB103A; BD-3; HBD3; HBP3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103; BD3; DEFB103; DEFB3 DEFB103B ; BD-3; DEFB-3; HBD3; hBD-3

D103A_HUMAN

Human

67

This assay has high sensitivity and excellent specificity for detection of human beta -defensins 3. No significant cross-reactivity or interference between human beta -defensins 3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 31.25 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

125656159

NP_001075020.1

NM_001081551.3

P81534

7,697 Da

Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans. [provided by RefSeq, Oct 2014]

Function: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.1 PublicationManual assertion based on experiment in:Ref.1

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800