catalog number :
MBS700452
products type :
ELISA Kit
products full name :
Dog Erythropoietin, EPO ELISA Kit
products short name :
erythropoietin
products name syn :
Dog Erythropoietin; EPO ELISA Kit; EP; MGC138142; MVCD2; epoetin; erythropoietin
other names :
erythropoietin; Erythropoietin; erythropoietin
other gene names :
EPO; EPO
uniprot entry name :
EPO_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog EPO. No significant cross-reactivity or interference between Dog EPO and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Erythrocyte maturation
products description :
For Sample For the quantitative determination of dog erythropoietin (EPO) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for erythropoietin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any erythropoietin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for erythropoietin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of erythropoietin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001006647.1
ncbi gb acc num :
NM_001006646.1
ncbi mol weight :
22,666 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198617); HIF-1 Signaling Pathway (695209); Hematopoietic Cell Lineage Pathway (84128); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (84127); Jak-STAT Signaling Pathway (488); PI3K-Akt Signaling Pathway (692237); PI3K-Akt Signaling Pathway (692979)
uniprot summary :
Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
size4 :
10x96-Strip-Wells