catalog number :
MBS700432
products type :
ELISA Kit
products full name :
Rat neuropeptide Y, NPY ELISA Kit
products short name :
neuropeptide Y
products name syn :
Rat neuropeptide Y (NPY) ELISA kit; PYY4; OTTHUMP00000201946; OTTHUMP00000201947; neuropeptide Y
other names :
Neuropeptide Y; Neuropeptide Y
other gene names :
NPY; NPY
uniprot entry name :
NPY_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat NPY. No significant cross-reactivity or interference between Rat NPY and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56pg/ml-100 pg/ml. Sensitivity: 0.39 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique.Antibody specific for NP-Y has been pre-coated onto a microplate. Standardsand samples are pipetted into the wells and any NP-Y present is bound by theimmobilized antibody. After removing any unbound substances, abiotin-conjugated antibody specific for NP-Y is added to the wells. After washing,avidin conjugated Horseradish Peroxidase (HRP) is added to the wells.Following a wash to remove any unbound avidin-enzyme reagent, a substratesolution is added to the wells and color develops in proportion to the amount ofNP-Y bound in the initial step. The color development is stopped and theintensity of the color is measured.
ncbi mol weight :
4,273 Da
ncbi pathways :
Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)
uniprot summary :
Function: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone
size4 :
10x96-Strip-Wells