catalog number :
MBS700431
products type :
ELISA Kit
products full name :
Human Vasoactive Intestinal Peptide, VIP ELISA Kit
products short name :
[vasoactive intestinal peptide]
products name syn :
[Human Vasoactive Intestinal Peptide; VIP ELISA Kit; MGC13587; PHM27; ; vasoactive intestinal peptide]
other names :
[VIP peptides isoform 1 preproprotein; VIP peptides; VIP peptides; prepro-VIP; vasoactive intestinal peptide; Peptide histidine valine 42]
products gene name :
[VIP]
other gene names :
[VIP; VIP; PHM27; VIP]
uniprot entry name :
VIP_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human VIP. No significant cross-reactivity or interference between human VIP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human VIP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_003372.1
ncbi gb acc num :
NM_003381.3
ncbi mol weight :
19,169 Da
ncbi pathways :
G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
ncbi summary :
The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.1 PublicationManual assertion based on experiment in:Ref.13
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells