ELISA/assay

Human gastrin-reliasing peptide, GRP ELISA Kit

MBS700420

48-Strip-Wells

565 USD

ELISA Kit

Human gastrin-reliasing peptide, GRP ELISA Kit

gastrin-releasing peptide

Human gastrin-reliasing peptide; GRP ELISA Kit; BN; GRP-10; preproGRP; proGRP; bombesin; neuromedin C; pre-progastrin releasing peptide; gastrin-releasing peptide

gastrin-releasing peptide isoform 2 preproprotein; Gastrin-releasing peptide; gastrin-releasing peptide; bombesin; prepro-GRP; neuromedin C; pre-progastrin releasing peptide; gastrin-releasing peptide; GRP-10

GRP

GRP; GRP; BN; GRP-10; proGRP; preproGRP; GRP

GRP_HUMAN

Human

141

This assay has high sensitivity and excellent specificity for detection of human GRP. No significant cross-reactivity or interference between human GRP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml -80 ng/ml. Sensitivity: 0.312 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

60498997

NP_001012530.1

NM_001012512.1

P07492

16,213 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Function: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP-receptor gene expression, long-term potentiation, and amygdala-dependent memory for fear

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150