ELISA/assay

Mouse Aromatase, ARO ELISA Kit

MBS700419

48-Strip-Wells

510 USD

ELISA Kit

Mouse Aromatase, ARO ELISA Kit

cytochrome P450, family 19, subfamily A, polypeptide 1

Mouse Aromatase (ARO) ELISA Kit; ARO; ARO1; CPV1; CYAR; CYP19; MGC104309; P-450AROM; OTTHUMP00000162543; aromatase; cytochrome P450; subfamily XIX (aromatization of androgens) ; estrogen synthetase; flavoprotein-linked monooxygenase; mic; cytochrome P450; family 19; subfamily A; polypeptide 1

aromatase; Aromatase; aromatase; CYPXIX; estrogen synthase; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, 19, aromatase; cytochrome P450, family 19, subfamily a, polypeptide 1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase

CYP19A1

Cyp19a1; Cyp19a1; Ar; ArKO; Int5; Cyp19; Int-5; p450arom; Arom; Cyp19

CP19A_MOUSE

Mouse

503

This assay has high sensitivity and excellent specificity for detection of mouse ARO. No significant cross-reactivity or interference between mouse ARO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: The minimum detectable dose of mouse ARO is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARO bound in the initial step. The color development is stopped and the intensity of the color is measured.

6681099

NP_031836.1

NM_007810.3

P28649

58,015 Da

Biological Oxidations Pathway (970688); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (421781); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (468268); Cytochrome P450 Pathway (198390); Cytochrome P450 - Arranged By Substrate Type Pathway (971395); Endogenous Sterols Pathway (1001152); Estrogen Biosynthesis Pathway (1001066); Metabolic Pathways (132962); Metabolism Pathway (971233); Metabolism Of Lipids And Lipoproteins Pathway (970615)

Function: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Catalytic activity: Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins. Cofactor: Heme group

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800