Dog inducible nitric oxide synthase, iNOS ELISA Kit | MyBioSource MBS700418 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Dog inducible nitric oxide synthase, iNOS ELISA Kit

catalog :

MBS700418

quantity :

96 Strip Wells

price :

915 USD

product information

catalog number :

MBS700418

products type :

ELISA Kit

products full name :

Dog inducible nitric oxide synthase, iNOS ELISA Kit

products short name :

nitric oxide synthase 2, inducible

products name syn :

Dog inducible nitric oxide synthase; iNOS ELISA Kit; HEP-NOS; INOS; NOS; NOS2A; NOS; type II; nitric oxide synthase 2A; nitric oxide synthase 2A (inducible; hepatocytes) ; nitric oxide synthase; macrophage; nitric oxide synthase 2; inducible

other names :

nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes); Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

products gene name :

NOS2

other gene names :

NOS2; NOS2; INOS; NOS2A; Inducible NOS; iNOS

uniprot entry name :

NOS2_CANFA

reactivity :

Dog

sequence length :

1154

specificity :

This assay has high sensitivity and excellent specificity for detection of Dog NOS2. No significant cross-reactivity or interference between Dog NOS2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 2.5 IU/ml-160 IU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.625 U/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: Nitric oxide synthases (NOSs) are present among eukaryoticenzymes as dimeric, calmodulin-dependent orcalmodulin-containing cytochrome p450-like hemoprotein thatcombine reductase and oxygenase catalytic domains in onedimer, bear both flavin adenine dinucleotide (FAD) and flavinmononucleotide (FMN), and carry out a 5`-electron oxidation ofnon-aromatic amino acid L-arginine with the aid oftetrahydrobiopterin. NOS is an enzyme in the body thatcontributes to transmission from one neuron to another, to theimmune system and to dilating blood vessels. It does so bysynthesis of nitric oxide from the terminal nitrogen atom ofL-arginine in the presence of NADPH and dioxygen. NOS is theonly known enzyme that binds FAD, FMN, heme,tetrahydrobiopterin (BH4) and calmodulin.There are three known isoforms, two are constitutive (cNOS) andthe third is inducible (iNOS). As opposed to the criticalcalcium-dependent regulation of constitutive NOS enzymes(nNOS and eNOS), iNOS has been described ascalcium-insensitive, likely due to its tight non-covalent interactionwith calmodulin and Ca2+. While evidence for 'baseline' iNOS expression has been elusive, IRF-1 and NF-kappaB-dependentactivation of the inducible NOS promoter supports aninflammation mediated stimulation of this transcript. From afunctional perspective, it is important to recognize that inductionof the high-output iNOS usually occurs in an oxidativeenvironment, and thus high levels of NO have the opportunity toreact with superoxide leading to peroxynitrite formation and celltoxicity. These properties may define the roles of iNOS in hostimmunity, enabling its participation in anti-microbial andanti-tumor activities as part of the oxidative burst ofmacrophages. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to iNOS. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for iNOS andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain iNOS, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of iNOS in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

ncbi gi num :

50978930

ncbi acc num :

NP_001003186.1

ncbi gb acc num :

NM_001003186.1

uniprot acc num :

O62699

ncbi mol weight :

131,847 Da

ncbi pathways :

Amoebiasis Pathway 167342!!Amoebiasis Pathway 167191!!Arginine And Proline Metabolism Pathway 84003!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 84100!!Calcium Signaling Pathway 459!!Chagas Disease (American Trypanosomiasis) Pathway 147812!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Disease Pathway 1016552!!HIF-1 Signaling Pathway 695209

uniprot summary :

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 . Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . Enzyme regulation: Regulated by calcium/calmodulin

size :

96 Strip Wells

price :

915 USD