ELISA/assay

Human antidiuretic hormone/vasopressin/arginine vasopressin, ADH/VP/AVP ELISA Kit

MBS700417

48-Strip-Wells

565 USD

ELISA Kit

Human antidiuretic hormone/vasopressin/arginine vasopressin, ADH/VP/AVP ELISA Kit

arginine vasopressin

Human antidiuretic hormone/vasopressin/arginine vasopressin; ADH/VP/AVP ELISA Kit; ADH; ARVP; AVP-NPII; AVRP; VP; antidiuretic hormone; arginine vasopressin-neurophysin II; neurohypophyseal; vasopressin-neurophysin II-copeptin; arginine vasopressin

vasopressin-neurophysin 2-copeptin preproprotein; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; arginine vasopressin; AVP-NPIICleaved into the following 3 chains:Arg-vasopressinAlternative name(s):Arginine-vasopressin

AVP

AVP; AVP; VP; ADH; ARVP; AVRP; AVP-NPII; ARVP; VP

NEU2_HUMAN

Human

164

This assay has high sensitivity and excellent specificity for detection of human ADH/VP/AVP. No significant cross-reactivity or interference between human ADH/VP/AVP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 pg/ml -80 pg/ml. Sensitivity: The minimum detectable dose of human ADH/VP/AVP is typically less than 0.31 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADH/VP/AVP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADH/VP/AVP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH/VP/AVP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH/VP/AVP bound in the initial step. The color development is stopped and the intensity of the color is measured.

13259533

NP_000481.2

NM_000490.4

P01185

17,325 Da

Aquaporin-mediated Transport Pathway (187187); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358)

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Function: Neurophysin 2 specifically binds vasopressin.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150