Human transient receptor potential Cation channel subfamily V member 4, TrpV4 ELISA Kit | MyBioSource MBS700415 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human transient receptor potential Cation channel subfamily V member 4, TrpV4 ELISA Kit

catalog :

MBS700415

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700415

products type :

ELISA Kit

products full name :

Human transient receptor potential Cation channel subfamily V member 4, TrpV4 ELISA Kit

products short name :

transient receptor potential cation channel, subfamily V, member 4

products name syn :

Human transient receptor potential cation channel subfamily V member 4 (TrpV4) ELISA Kit; OTRPC4; TRP12; VR-OAC; VRL-2; VRL2; VROAC; OSM9-like transient receptor potential channel 4; osmosensitive transient receptor potential channel 4; transient receptor potential channel 12; vanilloid rec; transient receptor potential cation channel; subfamily V; member 4

other names :

transient receptor potential cation channel subfamily V member 4 isoform e; Transient receptor potential cation channel subfamily V member 4; transient receptor potential cation channel subfamily V member 4; osm-9-like TRP channel 4; vanilloid receptor-like channel 2; transient receptor potential protein 12; OSM9-like transient receptor potential channel 4; osmosensitive transient receptor potential channel 4; vanilloid receptor-related osmotically activated channel; transient receptor potential cation channel, subfamily V, member 4; Osm-9-like TRP channel 4; OTRPC4; Transient receptor potential protein 12; TRP12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; VRL-2; Vanilloid receptor-related osmotically-activated channel; VR-OAC

products gene name :

TRPV4

other gene names :

TRPV4; TRPV4; SMAL; VRL2; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1; VRL2; VROAC; TrpV4; OTRPC4; TRP12; VRL-2; VR-OAC

uniprot entry name :

TRPV4_HUMAN

reactivity :

Human

sequence length :

824

specificity :

This assay has high sensitivity and excellent specificity for detection of human Trp V4. No significant cross-reactivity or interference between human Trp V4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TrpV4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TrpV4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TrpV4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TrpV4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

294459965

ncbi acc num :

NP_001170899.1

ncbi gb acc num :

NM_001177428.1

uniprot acc num :

Q9HBA0

ncbi mol weight :

98,281 Da

ncbi pathways :

Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Ion Channel Transport Pathway (187193); Stimuli-sensing Channels Pathway (771568); TRP Channels Pathway (906028); Transmembrane Transport Of Small Molecules Pathway (106572)

ncbi summary :

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

uniprot summary :

Function: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca2+ in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.3 PublicationsManual assertion based on experiment in:Ref.2

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage