Human growth-regulated oncogenealpha/melanoma growth stimulating activity, GROalpha/MGSA ELISA Kit | MyBioSource MBS700409 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human growth-regulated oncogenealpha/melanoma growth stimulating activity, GROalpha/MGSA ELISA Kit

catalog :

MBS700409

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700409

products type :

ELISA Kit

products full name :

Human growth-regulated oncogenealpha/melanoma growth stimulating activity, GROalpha/MGSA ELISA Kit

products short name :

chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

products name syn :

Human growth-regulated oncogenealpha/melanoma growth stimulating activity; GROalpha/MGSA ELISA Kit; FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1; GRO1 oncogene (melanoma growth stimulating activity; alpha) ; GRO1 oncogene (melanoma growth-stimulating activity) ; MGSA alpha; chemokine (C-X-C motif) ligan; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha)

other names :

growth-regulated alpha protein; Growth-regulated alpha protein; growth-regulated alpha protein; MGSA alpha; GRO-alpha(1-73); C-X-C motif chemokine 1; fibroblast secretory protein; neutrophil-activating protein 3; melanoma growth stimulatory activity alpha; GRO1 oncogene (melanoma growth-stimulating activity); GRO1 oncogene (melanoma growth stimulating activity, alpha); chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); C-X-C motif chemokine 1; GRO-alpha(1-73); Melanoma growth stimulatory activity; MGSA; Neutrophil-activating protein 3; NAP-3

products gene name :

CXCL1

other gene names :

CXCL1; CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a; GRO; GRO1; GROA; MGSA; SCYB1; MGSA; NAP-3

uniprot entry name :

GROA_HUMAN

reactivity :

Human

sequence length :

107

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CXCL1. No significant cross-reactivity or interference between Human CXCL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, cell culture supernates, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Inflammatory response

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GROalpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GROalpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GROalpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GROalpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4504153

ncbi acc num :

NP_001502.1

ncbi gb acc num :

NM_001511.3

uniprot acc num :

P09341

ncbi mol weight :

11,301 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102)

ncbi summary :

This gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signal through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Jan 2012]

uniprot summary :

Function: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.2 PublicationsManual assertion based on experiment in:Ref.6

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage