Mouse matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit | MyBioSource MBS700408 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

catalog :

MBS700408

quantity :

96 Strip Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700408

products type :

ELISA Kit

products full name :

Mouse matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

products short name :

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

products name syn :

Mouse matrix metalloproteinase 2/Gelatinase A; MMP-2 ELISA kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase)

other names :

72 kDa type IV collagenase; 72 kDa type IV collagenase; 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2; matrix metallopeptidase 2; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2

products gene name :

MMP2

other gene names :

Mmp2; Mmp2; GelA; Clg4a; MMP-2; MMP-2

uniprot entry name :

MMP2_MOUSE

reactivity :

Mouse

sequence length :

662

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse MMP2. No significant cross-reactivity or interference between Mouse MMP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Introduction: 72 kDa type IV collagenase also known as matrixmetalloproteinase-2 (MMP-2) and gelatinase A is an enzyme thatin humans is encoded by the MMP2 gene.Proteins of the matrix metalloproteinase (MMP) family are involvedin the breakdown of extracellular matrix in normal physiologicalprocesses, such as embryonic development, reproduction, andtissue remodeling, as well as in disease processes, such asarthritis and metastasis. Most MMP's are secreted as inactiveproproteins which are activated when cleaved by extracellularproteinases. This gene encodes an enzyme which degrades typeIV collagen, the major structural component of basementmembranes. The enzyme plays a role in endometrial menstrualbreakdown, regulation of vascularization and the inflammatoryresponse. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are then addedto the appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for MMP-2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate welland incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine)substrate solution is added to each well. Only those wells thatcontain MMP-2, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of MMP-2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

ncbi gi num :

6678902

ncbi acc num :

NP_032636.1

ncbi gb acc num :

NM_008610.2

uniprot acc num :

P33434

ncbi mol weight :

74,102 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway 1001029!!Axon Guidance Pathway 970585!!Bladder Cancer Pathway 83308!!Bladder Cancer Pathway 527!!Collagen Degradation Pathway 1001030!!Degradation Of The Extracellular Matrix Pathway 1001028!!Developmental Biology Pathway 970813!!EPH-Ephrin Signaling Pathway 1000783!!EPH-ephrin Mediated Repulsion Of Cells Pathway 1000787!!Estrogen Signaling Pathway 799184

uniprot summary :

Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues 1 PublicationManual assertion based on experiment in:Ref.4. Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly- -Ile-Ala-Gly-Gln. Cofactor: Binds 4 calcium ions per subunit

size :

96 Strip Wells

price :

575 USD

more info or order :

MyBioSource product webpage