ELISA/assay

Human mammalian target of rapamyein, mTOR ELISA Kit

MBS700406

48-Strip-Wells

565 USD

ELISA Kit

Human mammalian target of rapamyein, mTOR ELISA Kit

mammalian target of rapamyein, mTOR

Human mammalian target of rapamyein; mTOR ELISA Kit; mammalian target of rapamyein; mTOR

mammalian target of rapamycin; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin (serine/threonine kinase); mammalian target of rapamycin; Mammalian target of rapamycin

mTOR

MTOR; mTOR

D9I5T4_CAPHI

Human

2549

This assay has high sensitivity and excellent specificity for detection of human mTOR. No significant cross-reactivity or interference between human mTOR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of human mTOR is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mTOR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mTOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mTOR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mTOR bound in the initial step. The color development is stopped and the intensity of the color is measured.

297613711

ADI48287.1

D9I5T4

288,647 Da

AMPK Signaling Pathway (990729); AMPK Signaling Pathway (992181); Acute Myeloid Leukemia Pathway (916173); Acute Myeloid Leukemia Pathway (529); Adipocytokine Signaling Pathway (916110); Adipocytokine Signaling Pathway (505); ErbB Signaling Pathway (916024); ErbB Signaling Pathway (458); Glioma Pathway (916166); Glioma Pathway (522)

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150