ELISA/assay

Mouse Soluble Cluster of differentiation 40 ligand, sCD40L ELISA Kit

MBS700402

48-Strip-Wells

510 USD

ELISA Kit

Mouse Soluble Cluster of differentiation 40 ligand, sCD40L ELISA Kit

CD40 ligand

Mouse Soluble Cluster of differentiation 40 ligand; sCD40L ELISA Kit; RP3-527F8.3; CD154; CD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; gp39; hCD40L; CD40 antigen ligand; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfa; CD40 ligand

CD40 ligand; CD40 ligand; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form

CD40LG

Cd40lg; Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Cd40l; Tnfsf5; CD40-L; TRAP

CD40L_MOUSE

Mouse

260

This assay has high sensitivity and excellent specificity for detection of Mouse CD40LG. No significant cross-reactivity or interference between Mouse CD40LG and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to sCD40L. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for sCD40L andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain sCD40L, biotin-conjugatedantibody and enzyme-conjugated Avidin will exhibit a change incolor. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change ismeasured spectrophotometrically at a wavelength of 450 nm +/- 2nm. The concentration of sCD40L in the samples is thendetermined by comparing the O.D. of the samples to thestandard curve.

15011846

NP_035746.2

NM_011616.2

P27548

29,370 Da

Adaptive Immune System Pathway (971210); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Asthma Pathway (83313); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine-cytokine Receptor Interaction Pathway (83248)

Function: Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800