ELISA/assay

Human Apelin ELISA Kit

MBS700401

48-Strip-Wells

565 USD

ELISA Kit

Human Apelin ELISA Kit

apelin

Human Apelin ELISA kit; XNPEP2; apelin; AGTRL1 ligand; peptide ligand for APJ receptor; apelin

apelin preproprotein; Apelin; apelin; AGTRL1 ligand; APJ endogenous ligand; apelin; APJ endogenous ligandCleaved into the following 4 chains:Apelin-36; Apelin-31; Apelin-28; Apelin-13

APLN

APLN; APLN; APEL; XNPEP2; APEL

APEL_HUMAN

Human

77

This assay has high sensitivity and excellent specificity for detection of human apelin. No significant cross-reactivity or interference between human apelin and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Concentrations in serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 125 pg/ml-8000 pg/ml. Sensitivity: Typically less than 31.25 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for apelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any apelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for apelin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of apelin bound in the initial step. The color development is stopped and the intensity of the color is measured.

45120090

NP_059109.3

NM_017413.4

Q9ULZ1

8,569 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.[provided by RefSeq, Feb 2010]

Function: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.1 PublicationManual assertion based on experiment in:Ref.6

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150