ELISA/assay

Human Cathepsin K, Cath-K ELISA Kit

MBS700377

48-Strip-Wells

565 USD

ELISA Kit

Human Cathepsin K, Cath-K ELISA Kit

cathepsin K

Human Cathepsin K; cath-K ELISA Kit; RP11-363I22.4; CTS02; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; cathepsin O1; cathepsin O2; cathepsin X; cathepsin K

cathepsin K preproprotein; Cathepsin K; cathepsin K; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2; cathepsin K; Cathepsin O; Cathepsin O2; Cathepsin X

CTSK

CTSK; CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2; CTSO; CTSO2

CATK_HUMAN

Human

329

This assay has high sensitivity and excellent specificity for detection of human cath-K. No significant cross-reactivity or interference between human cath-K and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cath-K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cath-K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cath-K is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cath-K bound in the initial step. The color development is stopped and the intensity of the color is measured.

4503151

NP_000387.1

NM_000396.3

P43235

36,966 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Adaptive Immune System Pathway (366160); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Immune System Pathway (106386); Innate Immune System Pathway (106387); Lysosome Pathway (99052); Lysosome Pathway (96865); MHC Class II Antigen Presentation Pathway (645290)

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Function: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Catalytic activity: Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150