Rabbit anti-human Troponin T, cardiac muscle polyclonal Antibody | MyBioSource MBS7003657 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-human Troponin T, cardiac muscle polyclonal Antibody

catalog :

MBS7003657

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. PositiveWB detected in: Mouse skeletal muscle All. lanes: TNNT2 antibody at 4 ug/ml Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36,35,32,31 kDa. Observed bandsize:36,32kDa

image 2 :

IHC image of MBS7003657 dilutedat 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica Bond TMsystem. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer(pH6.0).Section was blocked with 10% normalgoatserum30min atRT.Then primary antibody(1%BSA)was incubated at 4°Covernight.The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SPsystem.

image 3 :

Immunofluorescence staining of Hela cells with MBS7003657 at 1:66,counter-stained withDAPI. The cells were fixedin4% formaldehyde, permeabilized using 0.2% TritonX-100 and blockedin10%normal GoatSerum.The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was AlexaFluor488-congugated Affini Pure Goat Anti-RabbitIgG(H+L).

product information

catalog number :

MBS7003657

products type :

Antibody

products full name :

Rabbit anti-human Troponin T, cardiac muscle polyclonal Antibody

products short name :

[Troponin T, cardiac muscle]

products name syn :

[Troponin T, cardiac muscle protein; Troponin T; cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT; TNNT2]

other names :

[troponin T, cardiac muscle isoform 1; Troponin T, cardiac muscle; troponin T, cardiac muscle; troponin T2, cardiac type; Cardiac muscle troponin T; cTnT]

products gene name syn :

[TNNT2]

other gene names :

[TNNT2; TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6; TnTc; cTnT]

uniprot entry name :

TNNT2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, mouse

sequence length :

295

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

tested application :

ELISA (EIA) , Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB:1:1000-1:5000. IHC:1:20-1:200. IF:1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant Human TroponinT, cardiac muscle protein (138-298AA). Relevance: Troponin T is the tropomyosin-binding subunit of troponin,the thin filament regulatory complex which confers calcium-sensitivity to striated muscleactomyosin ATPase activity.

other info2 :

Conjugation: Non-conjugated. Storage Buffer: 0.1 mol/ LNaCL, 15 mmol/LNaN3,PH 7.2. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8121 / sc-52289

products categories :

Cardiovascular

products description :

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. This protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.

ncbi gi num :

48255877

ncbi acc num :

NP_000355.2

ncbi gb acc num :

NM_000364.3

uniprot acc num :

P45379

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (1269868)

ncbi summary :

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Motor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: cytosol; sarcomere; striated muscle thin filament; troponin complex. Molecular Function: actin binding; ATPase activity; protein binding; protein binding, bridging; structural constituent of cytoskeleton; tropomyosin binding; troponin C binding; troponin I binding. Biological Process: actin crosslink formation; atrial cardiac muscle morphogenesis; cardiac muscle contraction; muscle filament sliding; negative regulation of ATPase activity; positive regulation of ATPase activity; protein heterooligomerization; regulation of heart contraction; regulation of muscle filament sliding speed; response to calcium ion; sarcomere organization; ventricular cardiac muscle morphogenesis. Disease: Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2; Cardiomyopathy, Familial Restrictive, 3

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage