catalog number :
MBS700351
products type :
ELISA Kit
products full name :
Human Matrix Gla Protein, MGP ELISA Kit
products short name :
[matrix Gla protein]
products name syn :
[Human Matrix Gla Protein; MGP ELISA Kit; GIG36; MGLAP; NTI; ; matrix Gla protein]
other names :
[matrix Gla protein isoform 2; Matrix Gla protein; matrix Gla protein; cell growth-inhibiting gene 36 protein; matrix Gla protein; Cell growth-inhibiting gene 36 protein]
products gene name :
[MGP]
other gene names :
[MGP; MGP; NTI; GIG36; MGLAP; MGLAP; GIG36; MGP]
uniprot entry name :
MGP_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human MGLL. No significant cross-reactivity or interference between human MGLL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 39.06 pg/ml-2500 pg/ml. Sensitivity: 9.76 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MGLL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MGLL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGLL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGLL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_000891.2
ncbi gb acc num :
NM_000900.3
ncbi mol weight :
12,353 Da
ncbi pathways :
Endochondral Ossification Pathway (198812); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)
ncbi summary :
The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
uniprot summary :
Function: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells