Human adenosine deaminase, ADA ELISA Kit | MyBioSource MBS700350 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human adenosine deaminase, ADA ELISA Kit

catalog :

MBS700350

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS700350

products type :

ELISA Kit

products full name :

Human adenosine deaminase, ADA ELISA Kit

products short name :

[adenosine deaminase]

products name syn :

[Human adenosine deaminase; ADA ELISA Kit; adenosine aminohydrolase; adenosine deaminase]

other names :

[adenosine deaminase; Adenosine deaminase; adenosine deaminase; adenosine aminohydrolase; adenosine deaminase; Adenosine aminohydrolase]

products gene name :

[ADA]

other gene names :

[ADA; ADA; ADA1]

uniprot entry name :

ADA_HUMAN

reactivity :

Human

sequence length :

363

specificity :

This assay has high sensitivity and excellent specificity for detection of human ADA. No significant cross-reactivity or interference between human ADA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 mIU/ml -100 mIU/ml. Sensitivity: The minimum detectable dose of human ADA is typically less than 0.39 mlU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

47078295

ncbi acc num :

NP_000013.2

ncbi gb acc num :

NM_000022.2

uniprot acc num :

P00813

ncbi mol weight :

40,764 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Purine Salvage Pathway (106273)

ncbi summary :

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.1 PublicationManual assertion based on experiment in:Ref.13. Catalytic activity: Adenosine + H2O = inosine + NH3.UniRule annotationHAMAP-Rule:MF_00540. Cofactor: Binds 1 zinc ion per subunit.1 PublicationManual assertion based on experiment in:Ref.18

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465