Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit | MyBioSource MBS700348 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit

catalog :

MBS700348

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS700348

products type :

ELISA Kit

products full name :

Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit

products short name :

[runt-related transcription factor 2]

products name syn :

[Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit; RP1-166H4.1; AML3; CBFA1; CCD; CCD1; MGC120022; MGC120023; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; runt-related transcription factor 2]

other names :

[runt-related transcription factor 2 isoform b; Runt-related transcription factor 2; runt-related transcription factor 2; PEA2-alpha A; PEBP2-alpha A; oncogene AML-3; acute myeloid leukemia 3 protein; SL3-3 enhancer factor 1 alpha A subunit; osteoblast-specific transcription factor 2; SL3/AKV core-binding factor alpha A subunit; core-binding factor, runt domain, alpha subunit 1; polyomavirus enhancer-binding protein 2 alpha A subunit; runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit]

products gene name :

[RUNX2]

other gene names :

[RUNX2; RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1; AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A]

uniprot entry name :

RUNX2_HUMAN

reactivity :

Human

sequence length :

499

specificity :

This assay has high sensitivity and excellent specificity for detection of human CBFA1/RUNX2. No significant cross-reactivity or interference between human CBFA1/RUNX2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Quantitative Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: <7.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CBFA1/RUNX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBFA1/RUNX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBFA1/RUNX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBFA1/RUNX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

226442791

ncbi acc num :

NP_001015051.3

ncbi gb acc num :

NM_001015051.3

uniprot acc num :

Q13950

ncbi mol weight :

56,648 Da

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); BMP Signalling And Regulation Pathway (198910); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Interleukin-11 Signaling Pathway (698753); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); Regulation Of Retinoblastoma Protein Pathway (137916)

ncbi summary :

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) Inhibits KAT6B-dependent transcriptional activation.1 PublicationManual assertion based on experiment in:Ref.7

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465

more info or order :

MyBioSource product webpage