Rabbit anti-human Gelsolin polyclonal Antibody(GSN) | MyBioSource MBS7003440 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-human Gelsolin polyclonal Antibody(GSN)

catalog :

MBS7003440

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. Positive WB detected in: THP-1 whole cell lysate. All lanes: GSN antibody at 3.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 86, 81, 82 kDa. Observed band size: 86 kDa

image 2 :

IHC image of MBS7003440 diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica Bond system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

image 3 :

Immunofluorescence staining of Hela cells with MBS7003440 at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

product information

catalog number :

MBS7003440

products type :

Antibody

products full name :

Rabbit anti-human Gelsolin polyclonal Antibody(GSN)

products short name :

[Gelsolin]

products name syn :

[Gelsolin (AGEL) (Actin-depolymerizing factor) (ADF) (Brevin), GSN]

other names :

[gelsolin isoform a; Gelsolin; AGEL; Actin-depolymerizing factor; ADF; Brevin]

other gene names :

[GSN; ADF]

uniprot entry name :

GELS_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

tested application :

ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Recommended dilution: WB: 1:500-1:5000, IHC: 1:200-1:500, IF: 1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant Human Gelsolin protein (28-782AA). Conjugate: Non-conjugated. Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Species: Human. Research Area: Signal Transduction

other info2 :

Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6405 / sc-6406 / sc-22602 / sc-22600 / sc-48769

products description :

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (endblocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

ncbi gi num :

4504165

ncbi acc num :

NP_000168.1

ncbi gb acc num :

NM_000177.4

uniprot acc num :

P06396

ncbi pathways :

Amyloid Fiber Formation Pathway (1269169); Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1270289); Coregulation Of Androgen Receptor Activity Pathway (138085); EGF Receptor (ErbB1) Signaling Pathway (137970); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590)

uniprot summary :

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Nuclear receptor co-regulator; Actin-binding. Chromosomal Location of Human Ortholog: 9q33. Cellular Component: actin cap; actin cytoskeleton; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; ruffle; sarcoplasm. Molecular Function: actin binding; calcium ion binding; myosin II binding; protein binding; protein domain specific binding. Biological Process: actin filament capping; actin filament polymerization; actin filament severing; actin nucleation; aging; apoptosis; barbed-end actin filament capping; cell structure disassembly during apoptosis; cellular protein metabolic process; extracellular matrix disassembly; negative regulation of virion penetration into host cell; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; programmed cell death; protein destabilization; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration. Disease: Amyloidosis, Finnish Type

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage