ELISA/assay

Rat glucocorticoid receptor, GR ELISA Kit

MBS700327

96 Strip Wells

915 USD

ELISA Kit

Rat glucocorticoid receptor, GR ELISA Kit

nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Rat glucocorticoid receptor; GR ELISA Kit; GCCR; GCR; GR; GRL; glucocorticoid receptor; nuclear receptor subfamily 3; group C; member 1 (glucocorticoid receptor)

glucocorticoid receptor; Glucocorticoid receptor; glucocorticoid receptor; GR; glucocorticoid receptor alpha; nuclear receptor subfamily 3 group C member 1; Nuclear receptor subfamily 3 group C member 1

NR3C1

NR3C1; NR3C1; GRL; GR

GCR_RABIT

Rat

772

This assay has high sensitivity and excellent specificity for detection of Rat NR3C1. No significant cross-reactivity or interference between Rat NR3C1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GR bound in the initial step. The color development is stopped and the intensity of the color is measured.

126723281

NP_001075616.1

NM_001082147.1

P59667

84,863 Da

Adipogenesis Pathway 198832!!Androgen Receptor Signaling Pathway 198806!!BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway 477138!!Circadian Clock Pathway 187173!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!IL-2 Signaling Pathway 198885!!Neuroactive Ligand-receptor Interaction Pathway 83053

Function: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression

96 Strip Wells

915 USD