FFAR3 Antibody | MyBioSource MBS7003084 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FFAR3 Antibody

catalog :

MBS7003084

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot. All lanes: FFAR3 antibody at 0.5ug/ml+Mouse kidney tissue. Goat polyclonal to rabbit at 1/10000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.

image 2 :

Immunohistochemistry of paraffin-embedded human liver cancer using MBS7003084 at dilution of 1:100

image 3 :

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7003084 at dilution of 1:100

product information

catalog number :

MBS7003084

products type :

Antibody

products full name :

FFAR3 Antibody

products short name :

[FFAR3]

products name syn :

[Free fatty acid receptor 3; G-protein coupled receptor 41; FFAR3; GPR41]

other names :

[free fatty acid receptor 3; Free fatty acid receptor 3; free fatty acid receptor 3; free fatty acid receptor 3; G-protein coupled receptor 41]

products gene name :

[FFAR3]

other gene names :

[FFAR3; FFAR3; FFA3R; GPR41; GPR42; GPR41]

uniprot entry name :

FFAR3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, mouse

sequence length :

346

purity :

>95%,Protein G purified

form :

Liquid; . Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

storage stability :

Upon reeipt store at -20°C or -80°C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western Blot (WB) 1:500-1:2000. Immunohistochemistry (IHC): 1:20-1:200. Immunofluorescence (IF): 1:50-1:200.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescent analysis of HepG2 cells using MBS7003084 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

image5 heading :

Immunofluorescence (IF)

image5 description :

Immunofluorescent analysis of A549 cells using MBS7003084 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

other info1 :

Immunogen: Recombinant human Free fatty acid receptor 3 protein. Research Area: Cardiovascular

other info2 :

Conjugation: Non-conjugated. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-131161 / sc-131163 / sc-131166 / sc-98332

products description :

G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins. Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation (PubMed:12711604). Activated by SCFAs and by beta-hydroxybutyrate, a ketone body produced by the liver upon starvation, it inhibits N-type calcium channels and modulates the activity of sympathetic neurons through a signaling cascade involving the beta and gamma subunits of its coupled G protein, phospholipase C and MAP kinases. Thereby, it may regulate energy expenditure through the control of the sympathetic nervous system that controls for instance heart rate. Upon activation by SCFAs accumulating in the intestine, it may also signal to the brain via neural circuits which in turn would regulate intestinal gluconeogenesis. May also control the production of hormones involved in whole-body energy homeostasis. May for instance, regulate blood pressure through renin secretion. May also regulate secretion of the PYY peptide by enteroendocrine cells and control gut motility, intestinal transit rate, and the harvesting of energy from SCFAs produced by gut microbiota. May also indirectly regulate the production of LEP/Leptin, a hormone acting on the CNS to inhibit food intake, in response to the presence of short-chain fatty acids in the intestine. Finally, may also play a role in glucose homeostasis. Besides its role in energy homeostasis, may play a role in intestinal immunity. May mediate the activation of the inflammatory and immune response by SCFAs in the gut, regulating the rapid production of chemokines and cytokines by intestinal epithelial cells. Among SCFAs, the fatty acids containing less than 6 carbons, the most potent activators are probably propionate, butyrate and pentanoate while acetate is a poor activator (PubMed:12496283, PubMed:12711604).

ncbi gi num :

4885329

ncbi acc num :

NP_005295.1

ncbi gb acc num :

NM_005304.3

uniprot acc num :

O14843

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); Free Fatty Acid Receptors Pathway (1269569); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543)

uniprot summary :

FFAR3: Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5- trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate acetate formate. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; lipid binding. Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; inflammatory response; mucosal immune response; negative regulation of blood pressure; positive regulation of acute inflammatory response to non-antigenic stimulus; positive regulation of chemokine production; positive regulation of cytokine production during immune response; regulation of hormone biosynthetic process; regulation of norepinephrine secretion

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage