ELISA/assay

Human Vascular cell adhesion molecule 1, VCAM-1 ELISA Kit

MBS700298

96-Strip-Wells

565 USD

ELISA Kit

Human Vascular cell adhesion molecule 1, VCAM-1 ELISA Kit

vascular cell adhesion molecule 1

Human Vascular cell adhesion molecule 1; VCAM-1 ELISA kit; CD106; DKFZp779G2333; INCAM-100; MGC99561; CD106 antigen; Human Vascular cell adhesion molecule 1; VCAM-1

vascular cell adhesion protein 1 isoform a; Vascular cell adhesion protein 1; vascular cell adhesion molecule 1; INCAM-100; CD_antigen: CD106

VCAM1

VCAM1; VCAM1; CD106; INCAM-100; L1CAM; V-CAM 1; VCAM-1

VCAM1_HUMAN

Human

739

This assay has high sensitivity and excellent specificity for detection of human VCAM-1. No significant cross-reactivity or interference between human VCAM-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.563 ng/ml -100 ng/ml. Sensitivity: 0.611 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4507875

NP_001069.1

NM_001078.3

P19320

81,276 Da

Adaptive Immune System Pathway (366160); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Extracellular Matrix Organization Pathway (576262); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

96-Strip-Wells

565 USD

5x96-Strip-Wells

1725

10x96-Strip-Wells

3200