ELISA/assay

Human Interleukin 35, IL-35 ELISA Kit

MBS700294

48-Strip-Wells

565 USD

ELISA Kit

Human Interleukin 35, IL-35 ELISA Kit

Interleukin 35 (IL-35)

Human Interleukin 35 (IL-35) ELISA Kit; Interleukin 35 (IL-35)

IL-35

Human

This assay has high sensitivity and excellent specificity for detection of human IL-35. No significant cross-reactivity or interference between human IL-35 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, cell culture supernates, tissue homogenates and urineurineurineurineurine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg /ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-35 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-35 bound in the initial step. The color development is stopped and the intensity of the color is measured.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150