ELISA/assay

Rat visfatin ELISA Kit

MBS700290

96 Strip Wells

915 USD

ELISA Kit

Rat visfatin ELISA Kit

nicotinamide phosphoribosyltransferase

Rat visfatin ELISA Kit; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF; PBEF1; VF; VISFATIN; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; nicotinamide phosphoribosyltransferase

nicotinamide phosphoribosyltransferase; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase; visfatin; NAmPRTase; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1 homolog; nicotinamide phosphoribosyltransferase; Pre-B-cell colony-enhancing factor 1 homolog; PBEF; Visfatin

NAMPT

Nampt; Nampt; Pbef; Pbef1; Pbef1; NAmPRTase; Nampt; PBEF

NAMPT_RAT

Rat

491

This assay has high sensitivity and excellent specificity for detection of Rat NAMPT. No significant cross-reactivity or interference between Rat NAMPT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 3.12 ng/ml-200 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.78 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Alkaloid biosynthesis and metabolism. Protein Biological Process 3: Pyridine nucleotide biosynthesis

Introduction: Visfatin, an adipokine that is highly enriched in the visceral fat, has increased expression level in plasma during the development of obesity, implying its important role in obesity. Visfatin (also known as PBEF and Nampt), a 52 kDa cytoplasmic protein composed of 473-amino acid, expresses primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue. In addition to its role in obesity, visfatin also has other biological functions. Visfatin is a nicotinamide phosphoribosyltransferase (Nampt) enzyme that catalyzes first step in the biosynthesis of NAD from nicotinamide. Visfatin has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to visfatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for visfatin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain visfatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of visfatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

29293813

NP_808789.1

NM_177928.3

Q80Z29

55,438 Da

Adipogenesis Pathway 198479!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 1010371!!Circadian Clock Pathway 1010370!!Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway 1010563!!Defective BTD Causes Biotidinase Deficiency Pathway 1010591!!Defective CD320 Causes Methylmalonic Aciduria Pathway 1010588!!Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway 1010577!!Defective GIF Causes Intrinsic Factor Deficiency Pathway 1010580!!Defective HLCS Causes Multiple Carboxylase Deficiency Pathway 1010590!!Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway 1010579

has similarity to cytokines but is not secreted; localized to the nucleus and cytoplasm, changes in subcellular localization may be associated with the cell cycle [RGD, Feb 2006]

Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function . Catalytic activity: Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate.

96 Strip Wells

915 USD