catalog number :
MBS700286
products type :
ELISA Kit
products full name :
Mouse D-Lactate Dehydrogenase, D-LDH ELISA Kit
products short name :
lactate dehydrogenase D
products name syn :
Mouse D-Lactate Dehydrogenase; D-LDH ELISA Kit; MGC57726; D-lactate dehydrogenase; lactate dehydrogenase D
other names :
probable D-lactate dehydrogenase, mitochondrial; Probable D-lactate dehydrogenase, mitochondrial; probable D-lactate dehydrogenase, mitochondrial; DLD; lactate dehydrogenase D
products gene name :
LDHD
other gene names :
Ldhd; Ldhd; D8Bwg1320e; 4733401P21Rik; DLD; Lactate dehydrogenase D
uniprot entry name :
LDHD_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse LDHD. No significant cross-reactivity or interference between Mouse LDHD and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 4.69 mU/ml-300 mU/ml. Detection Wavelength: 450 nm. Sensitivity: 1.17 mU/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for D-LDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any D-LDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for D-LDH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of D-LDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_081846.3
ncbi gb acc num :
NM_027570.3
ncbi mol weight :
51,848 Da
ncbi pathways :
Metabolism Of Proteins Pathway 970583!!Mitochondrial Protein Import Pathway 1000412!!Pyruvate Metabolism Pathway 83197!!Pyruvate Metabolism Pathway 373!!Aerobic Respiration -- Electron Donors Reaction List Pathway 143067!!Methylglyoxal Degradation I Pathway 142951!!Methylglyoxal Degradation II Pathway 142953!!Methylglyoxal Degradation VI Pathway 542837