Human Growth Hormone Binding Protein, GHBP ELISA Kit | MyBioSource MBS700276 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Growth Hormone Binding Protein, GHBP ELISA Kit

catalog :

MBS700276

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700276

products type :

ELISA Kit

products full name :

Human Growth Hormone Binding Protein, GHBP ELISA Kit

products short name :

growth hormone receptor

products name syn :

Human Growth Hormone Binding Protein; GHBP ELISA Kit; GHBP; growth hormone binding protein; serum binding protein; somatotropin receptor; growth hormone receptor

other names :

growth hormone receptor isoform 1; Growth hormone receptor; growth hormone receptor; GH receptor; serum binding protein; somatotropin receptor; growth hormone binding protein; growth hormone receptor; Somatotropin receptorCleaved into the following chain:Growth hormone-binding protein; GH-binding protein; GHBPAlternative name(s):Serum-binding protein

products gene name :

GHR

other gene names :

GHR; GHR; GHBP; GH receptor; GH-binding protein; GHBP

uniprot entry name :

GHR_HUMAN

reactivity :

Human

sequence length :

638

specificity :

This assay has high sensitivity and excellent specificity for detection of human GHBP. No significant cross-reactivity or interference between human GHBP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 10 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human GHBP is typically less than 4 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human GHBP concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<20%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with biotin-BSA and Avidin. Standards and samples are pipetted into the wells with a biotin-conjugated antibody specific for GHBP. After washing, GHBP antibody conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHBP in sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503993

ncbi acc num :

NP_000154.1

ncbi gb acc num :

NM_000163.4

uniprot acc num :

P10912

ncbi mol weight :

71,500 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

Function: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage