product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat transforming growth factor alpha, TGF-alpha ELISA Kit
catalog :
MBS700274
quantity :
48-Strip-Wells
price :
465 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS700274
products type :
ELISA Kit
products full name :
Rat transforming growth factor alpha, TGF-alpha ELISA Kit
products short name :
transforming growth factor, alpha
products name syn :
Rat transforming growth factor alpha; TGF-alpha ELISA Kit; TFGA; transforming growth factor-alpha; transforming growth factor; alpha
other names :
Transforming growth factor alpha; Transforming growth factor alpha; EGF-like TGF; ETGF; TGF type 1
products gene name :
TGFA
other gene names :
TGFA; TGF-alpha; ETGF
uniprot entry name :
TGFA_RABIT
reactivity :
Rat
sequence length :
50
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat TGFA. No significant cross-reactivity or interference between Rat TGFA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
1351230
ncbi acc num :
P98138.1
uniprot acc num :
P98138
ncbi mol weight :
5,565 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro).
size1 :
48-Strip-Wells
price1 :
465 USD
size2 :
96-Strip-Wells
price2 :
655
size3 :
5x96-Strip-Wells
price3 :
2455
size4 :
10x96-Strip-Wells
price4 :
4595
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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