ELISA/assay

Melamine ELISA Kit

MBS700269

96 Strip Wells

545 USD

ELISA Kit

Melamine ELISA Kit

Melamine

Melamine ELISA Kit

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: milk, milk powder, feed and tissue (pork, liver, chicken). Detection Range: 5 ppb-405 ppb. Detection Wavelength: 450 nm. Sensitivity: 5 ppb

Sample Volume: 50-100ul

Introduction: Melamine (2,4,6-triamino-1,3,5-triazine) is used in the production of plastics, in finishers for paper, in fertilizer, as a flame retardant, and in the manufacture of wrinkle-free textiles. Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. The discovery of melamine in pet food, animal feed, milk and protein sources including wheat gluten, rice protein concentrate, and corn gluten created an urgent need for rapid methods for detecting melamine in food. The ELISA for melamine proved to be a useful alternative to more cumbersome methods. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A polyclonal antibody specific for Melamine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between HRP labeled Melamine and unlabeled Melamine (Standards or samples) with the pre-coated antibody specific for Melamine. The more the amount of Melamine in samples, the less the HRP labeled Melamine bound by pre-coated antibody. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Melamine bound in the initial step. The color development is stopped and the intensity of the color is measured.

96 Strip Wells

545 USD