catalog number :
MBS700268
products type :
ELISA Kit
products full name :
Bovine Albumin, Alb ELISA Kit
products short name :
albumin
products name syn :
Bovine Albumin (Alb) ELISA kit; DKFZp779N1935; PRO0883; PRO0903; PRO1341; cell growth inhibiting protein 42; growth-inhibiting protein 20; serum albumin; Bovine Albumin (Alb)
other names :
serum albumin preproprotein; Serum albumin; serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42; albumin
other gene names :
ALB; ALB; PRO0883; PRO0903; PRO1341; GIG20; GIG42; PRO0903; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341
uniprot entry name :
ALBU_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine ALB. No significant cross-reactivity or interference between Bovine ALB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.313 ug/ml-80 ug/ml. Detection Wavelength: 450 nm. Sensitivity: 0.148ug/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Metabolism
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BSA. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated antibody preparation specific for BSA and incubated. A competitive inhibition reaction is launched between BSA (Standards or samples) and pre-coated with BSA with the biotin-conjugated antibody preparation specific for BSA. The more amount of BSA in samples, the less pre-coated with BSA bound by biotin-conjugated antibody preparation specific for BSA. Then add the HRP-avidin to each well.The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of BSA in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_000468.1
ncbi gb acc num :
NM_000477.5
ncbi mol weight :
69,367 Da
ncbi pathways :
Bile Acid And Bile Salt Metabolism Pathway 106144!!Binding And Uptake Of Ligands By Scavenger Receptors Pathway 771599!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!HDL-mediated Lipid Transport Pathway 106158!!Hemostasis Pathway 106028!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Platelet Activation, Signaling And Aggregation Pathway 106034
ncbi summary :
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.1 PublicationManual assertion based on experiment in:Ref.32