ELISA/assay

Human Cross-linked Carboxy-terminal telopeptide of type I  collagen, CTX-I ELISA Kit

MBS700254

48-Strip-Wells

510 USD

ELISA Kit

Human Cross-linked Carboxy-terminal telopeptide of type I  collagen, CTX-I ELISA Kit

Cross-linked Carboxy-terminal telopeptide of type I  collagen, CTX-I

Human Cross-linked Carboxy-terminal telopeptide of type I collagen; CTX-I ELISA Kit; Cross-linked Carboxy-terminal telopeptide of type I collagen; CTX-I

CTX-I

Human

This assay has high sensitivity and excellent specificity for detection of human CTX-I. No significant cross-reactivity or interference between human CTX-I and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: Typically less than 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTX-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTX-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTX-I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTX-I bound in the initial step. The color development is stopped and the intensity of the color is measured.

Function: Basic protein that bind to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities on many other cells, including red blood cells. Interaction with sulfatides in the cell membrane induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation It binds to the integrin alpha-V/beta-3 with a moderate affinity. Inhibits protein kinases C.1 PublicationManual assertion based on experiment in:Ref.5

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800