ELISA/assay

Rat diamine oxidase, DAO ELISA Kit

MBS700248

96 Strip Wells

895 USD

ELISA Kit

Rat diamine oxidase, DAO ELISA Kit

D-amino-acid oxidase

Rat diamine oxidase (DAO) ELISA Kit; DAAO; DAMOX; MGC35381; OXDA; ; D-amino-acid oxidase

D-amino-acid oxidase; D-amino-acid oxidase; D-amino-acid oxidase; DAAO; DAMOX; D-amino acid oxidase

DAO

DAO; DAO; DAAO; DAMOX; DAO

OXDA_RABIT

Rat

347

This assay has high sensitivity and excellent specificity for detection of Rat DAO. No significant cross-reactivity or interference between Rat DAO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.78 mIU/ml-50 mIU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.195 mIU/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DAO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DAO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

126723217

NP_001075658.1

NM_001082189.1

P22942

38,590 Da

Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!D-Arginine And D-ornithine Metabolism Pathway 82972!!D-Arginine And D-ornithine Metabolism Pathway 341!!Glycine, Serine And Threonine Metabolism Pathway 82949!!Glycine, Serine And Threonine Metabolism Pathway 313!!Glyoxylate Metabolism Pathway 160990!!Metabolism Pathway 477135!!Metabolism Of Amino Acids And Derivatives Pathway 106169!!Peroxisome Pathway 131226

Function: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids . Catalytic activity: A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2. Cofactor: FAD.

96 Strip Wells

895 USD