product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Sheep insulin-like growth factor 1, IGF-1 ELISA Kit
catalog :
MBS700234
quantity :
96 Strip Wells
price :
915 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS700234
products type :
ELISA Kit
products full name :
Sheep insulin-like growth factor 1, IGF-1 ELISA Kit
products short name :
insulin-like growth factor 1 (somatomedin C)
products name syn :
Sheep insulin-like growth factor 1; IGF-1 ELISA Kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C)
other names :
insulin-like growth factor I; Insulin-like growth factor I; insulin-like growth factor I; somatomedin; Somatomedin
products gene name :
IGF1
other gene names :
IGF1; IGF1; IGF-I; IGF-I
uniprot entry name :
IGF1_SHEEP
reactivity :
Sheep
sequence length :
154
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep IGF-1. No significant cross-reactivity or interference between sheep IGF-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of sheep insulin-like growth factor 1 (IGF-1) concentrations in serum, plasma, tissue homogenates. Detection Range: 15.6 ng/ml-1000 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of sheep IGF-1 is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
57526353
ncbi acc num :
NP_001009774.1
ncbi gb acc num :
NM_001009774.3
uniprot acc num :
P10763
ncbi mol weight :
17,012 Da
ncbi pathways :
Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149
uniprot summary :
Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation
size :
96 Strip Wells
price :
915 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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