ELISA/assay

Human proteoglycan 4, PRG4 ELISA Kit

MBS700233

48-Strip-Wells

505 USD

ELISA Kit

Human proteoglycan 4, PRG4 ELISA Kit

proteoglycan 4

Human proteoglycan 4 (PRG4) ELISA Kit; GS1-174L6.2; CACP; FLJ32635; HAPO; JCAP; MSF; SZP; bG174L6.2; OTTHUMP00000033580; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor) ; lubricin; megakaryocyte stimulat; proteoglycan 4

proteoglycan 4 isoform B; Proteoglycan 4; proteoglycan 4; lubricin; superficial zone proteoglycan; megakaryocyte stimulating factor; megakaryocyte-stimulating factor; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor ); proteoglycan 4; Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanCleaved into the following chain:Proteoglycan 4 C-terminal part

PRG4

PRG4; PRG4; MSF; SZP; CACP; HAPO; JCAP; bG174L6.2; MSF; SZP

PRG4_HUMAN

Human

1363

This assay has high sensitivity and excellent specificity for detection of human PRG4. No significant cross-reactivity or interference between human PRG4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 9.37 ng/ml-600 ng/ml. Sensitivity: Typically less than 2.34 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

189181720

NP_001121180.1

NM_001127708.1

Q92954

151,077 Da

The protein encoded by this gene is a large proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.2 PublicationsManual assertion based on experiment in:Ref.6

48-Strip-Wells

505 USD

96-Strip-Wells

715

5x96-Strip-Wells

2530

10x96-Strip-Wells

4730