Human Interleukin 28B, IL-28B ELISA Kit | MyBioSource MBS700232 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin 28B, IL-28B ELISA Kit

catalog :

MBS700232

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700232

products type :

ELISA Kit

products full name :

Human Interleukin 28B, IL-28B ELISA Kit

products short name :

Interleukin 28B (IL-28B)

products name syn :

Human Interleukin 28B (IL-28B) ELISA kit; IFNL3; IL-28B; IL28C; interferon; lambda 3; interferon; lambda 4; interleukin 28B; interleukin 28C; Interleukin 28B (IL-28B)

other names :

interferon lambda-3; Interferon lambda-3; interferon lambda-3; IL-28C; IFN-lambda-3; IFN-lambda-4; interleukin 28C; interleukin-28B; interleukin-28C; cytokine Zcyto22; interferon lambda-4; interferon, lambda 4; interleukin 28B (interferon, lambda 3); interferon, lambda 3; Cytokine Zcyto22; Interleukin-28B; IL-28B; Interleukin-28C; IL-28C

products gene name :

IL-28B

other gene names :

IFNL3; IFNL3; IL28B; IL28C; IL-28B; IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C

uniprot entry name :

IFNL3_HUMAN

reactivity :

Human

sequence length :

196

specificity :

This assay has high sensitivity and excellent specificity for detection of human IL-28B. No significant cross-reactivity or interference between human IL-28B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of human IL-28B is typically less than 0.39 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-28B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-28B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-28B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-28B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

28144901

ncbi acc num :

NP_742151.2

ncbi gb acc num :

NM_172139.2

uniprot acc num :

Q8IZI9

ncbi mol weight :

21,706 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Type III Interferon Signaling Pathway (755437)

ncbi summary :

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage