Fish Interleukin 1beta (IL- 1beta) ELISA Kit | MyBioSource MBS700230 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Interleukin 1beta (IL- 1beta) ELISA Kit

catalog :

MBS700230

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700230

products type :

ELISA Kit

products full name :

Fish Interleukin 1beta (IL- 1beta) ELISA Kit

products short name :

Interleukin 1beta (IL- 1beta)

products name syn :

Interleukin 1beta (IL- 1beta); Interleukin 1b (IL- 1b); Fish Interleukin 1b (IL- 1b) ELISA Kit

other names :

interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; catabolin; OTTHUMP00000162031; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1, beta; Catabolin

products gene name :

IL- 1beta

products gene name syn :

IL- 1b

other gene names :

IL1B; IL1B; IL-1; IL1F2; IL1-BETA; IL1F2

uniprot entry name :

IL1B_HUMAN

reactivity :

Fish

specificity :

This assay has high sensitivity and excellent specificity for detection of fish IL-1beta. No significant cross-reactivity or interference between fish IL-1beta and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL- 1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL- 1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL- 1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL- 1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

10835145

ncbi acc num :

NP_000567.1

ncbi gb acc num :

NM_000576.2

uniprot acc num :

P01584

ncbi mol weight :

30,748 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076); Chagas Disease (American Trypanosomiasis) Pathway (147809)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

uniprot summary :

Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Ref.16. Subunit structure: Monomer. Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. Sequence similarities: Belongs to the IL-1 family.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage